Cell Growth Determination Kit, MTT based from MilliporeSigma

Product Specs
ItemCell Growth Determination Kit, MTT based
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberCGD1
Applicationsdetection, protein quantification, UV/Vis spectroscopy, cell analysis
Quantity1KT

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MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

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Website: www.SigmaAldrich.com/

(1) Pulsed Electromagnetic Fields Induce Skeletal Muscle Cell Repair by Sustaining the Expression of Proteins Involved in the Response to Cellular Damage and Oxidative StressInternational Journal of Molecular SciencesNovember 23, 2023Silvia Maiullari, Antonella Cicirelli, Angela Picerno, Francesca Giannuzzi, Loreto Gesualdo, Angela Notarnicola, Fabio Sallustio, Biagio Morettimeasured by the MTT Cell Proliferation Assay Kit, according to the manufacturer’s instructions (Cat.no CGD1; Sigma, St. Louis, MO, USA). Cells were plated in 6-well plates at 2 × 105 cells/well, and then they

(2) Synthesis, Characterization, and In Vitro Cytotoxicity Evaluation of Doxorubicin-Loaded Magnetite Nanoparticles on Triple-Negative Breast Cancer Cell LinesPharmaceuticsJune 17, 2023Jano Markhulia, Shalva Kekutia, Vladimer Mikelashvili, Liana Saneblidze, Tamar Tsertsvadze, Nino Maisuradze, Nino Leladze, Zsolt Czigány, László Almásythe initial 0.5 h, 24 h, and 48 h phases. The test was performed following the kit protocol (Sigma: CGD1-1KT) as described in Section 2.7. MTT assay. The concentrations of the samples used in the study and

(3) Anticancer Agents Derived from Cyclic Thiosulfonates: Structure-Reactivity and Structure-Activity RelationshipsChemMedChemJuly 19, 2022Ghilardi AF, Yaaghubi E, Ferreira RB, Law ME, Yang Y, Davis BJ, Schilson CM, Ghiviriga I, Roitberg AE, Law BK, et al.(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. MTT assays were carried out according to the manufacturer’s instructions (kit CGD1, Sigma-Aldrich, St. Louis, MO). Results represent the mean ± standard deviation of six replicate determinations

(4) Antioxidative NAC-Loaded Silk Nanoparticles with Opening Mucosal Tight Junctions for Nasal Drug Delivery: An In Vitro and In Vivo StudyPharmaceuticsJune 17, 2022Tze-Wen Chung, Ting-Ya Wu, Zheng-Yu Siah, Der-Zen Liucytotoxicity of cells for the SF and SF/NAC NPs, the viability of L929 cells was measured using an MTT assay (CGD1, Sigma, USA) after they were incubated with different concentrations of the NPs (e.g., 0~6000 μg/mL)

(5) Regression of Triple-Negative Breast Cancer in a Patient-Derived Xenograft Mouse Model by Monoclonal Antibodies against IL-12 p40 MonomerCellsJanuary 13, 2022Madhuchhanda Kundu, Sumita Raha, Avik Roy, Kalipada PahanHamster IgG (cat# IR-HT-GF) was obtained from Innovative Research (Novi, MI, USA). MTT assay kit (cat# CGD1), and LDH assay kit (cat# TOX7) were purchased from Sigma (St. Louis, MO, USA). TUNEL assay kit (cat#

(6) Amitriptyline blocks innate immune responses mediated by toll‐like receptor 4 and IL‐1 receptor: Preclinical and clinical evidence in osteoarthritis and goutBritish Journal of PharmacologyJanuary 1, 2022Eloi Franco‐Trepat, Ana Alonso‐Pérez, María Guillán‐Fresco, Alberto Jorge‐Mora, Antia Crespo‐Golmar, Miriam López‐Fagúndez, Andrés Pazos‐Pérez, Oreste Gualillo, Susana Belén Bravo, Rodolfo Gómez Bahamondebiochemical assays include Griess assay (Ref. MAK367; Merk Sigma‐Aldrich, Germany), MTT assay (Ref. CGD1‐1KT; Merk Sigma‐Aldrich, Germany) and malachite green assay (Ref. M689; Thermo Fisher Scientific, USA). Materials

(7) Anti-PD-1 Checkpoint Therapy Can Promote the Function and Survival of Regulatory T CellsJournal of immunology (Baltimore, Md. : 1950)November 15, 2021Vick SC, Kolupaev OV, Perou CM, Serody JS.48 hours. Cells were then harvested and cell death was determined using MTT Cell Growth Assay (Sigma CGD1) following manufacture’s protocols. ABT-199 dose response curve and IC50 was calculated using Prism

(8) Periprostatic adipose tissue promotes prostate cancer resistance to docetaxel by paracrine IGF‐1 upregulation of TUBB2B beta‐tubulin isoformThe ProstateMay 1, 2021Antonietta Liotti et al.following treatment using the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay (CGD1, Sigma) according to the manufacturer's protocol. 2.5 Western blot For western blot assays, cells

(9) The effects of myelin on macrophage activation are phenotypic specific via cPLA₂ in the context of spinal cord injury inflammationScientific reportsMarch 18, 2021Kopper TJ, Zhang B, Bailey WM, Bethel KE, Gensel JC.MTT-based cell growth determination kit according to the manufacturer’s instructions (Sigma-Aldrich CGD1-1KT)23. Briefly, 24 h before testing, N2A growth media was replaced with serum-free N2A media to induce

(10) Lymphatic muscle cells contribute to dysfunction of the synovial lymphatic system in inflammatory arthritis in miceArthritis research & therapyFebruary 19, 2021Liang Q, Zhang L, Xu H, Li J, Chen Y, Schwarz EM, Shi Q, Wang Y, Xing L.3–6 were used. Cell growth and apoptosis assays Cell growth was examined by an MTT assay (Sigma, # CGD1) according to the manufacturer’s instructions. In brief, cells were seeded at a density of 2.4 × 103cells/well

(11) Altered chondrocyte differentiation, matrix mineralization and MEK-Erk1/2 signaling in an INPPL1 catalytic knock-out mouse model of opsismodysplasiaAdvances in Biological RegulationMay 1, 2020Charles-Andrew Vande Catsyne et al.for Western blotting and immunodetection. The viability of Ship2+/+ primary chondrocytes was tested using a MTT assay (CGD1-1 KT, Sigma). 2.5. Micromass culture of primary chondrocytes and ATDC5 cells Primary chondrocytes, prepared

(12) Antitumor mechanism of MAP30 in bladder cancer T24 cells, and its potential toxic effects in miceCellular and Molecular BiologyApril 20, 2020Zhenduo Shi, Kun Pang, Wen Yang, Jiahe Zhou, Bingzheng Dong, Zhiguo Zhang, Yan Zhao, Conghui Han, Lin Haothe kit protocols. Materials and Methods MTT assay MTT assay was performed using MTT assay kits (CGD1, Sigma-Aldrich) in line with the kit protocols. Cells in logarithmic growth phase were seeded in 96well

(13) Human RAD51 paralogue RAD51C fosters repair of alkylated DNA by interacting with the ALKBH3 demethylaseNucleic acids researchDecember 16, 2019Mohan M, Akula D, Dhillon A, Goyal A, Anindya R.48 h. Later, media was removed, and 100 μl of 0.5 mg/ml MTT reagent (Cell Growth Determination Kit, CGD1, Sigma-Aldrich) was added to each well. The cultures were incubated for 4 h at 37°C in the dark. The

(14) Effects on the Caco-2 Cells of a Hypoglycemic Protein from Lupin Seeds in a Solution and Adsorbed on Polystyrene Nanoparticles to Mimic a Complex Food MatrixBiomoleculesOctober 14, 2019Alberto Barbiroli, Jessica Capraro, Serena Marulo, Marta Gamba, Alessio ScarafoniVitality Assays The viable cell number was determined using a Cell Growth Determination Kit (Merck, cat. CGD1) on a 96-well multiplate according to the manufacturer’s instructions. This method is based on a (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium

(15) Tankyrase disrupts metabolic homeostasis and promotes tumorigenesis by inhibiting LKB1-AMPK signallingNature communicationsSeptember 25, 2019Li N, Wang Y, Neri S, Zhen Y, Fong LWR, Qiao Y, Li X, Chen Z, Stephan C, Deng W, et al.3 days. Cell death was determined by MTT assay with a Cell Growth Determination Kit (Sigma–Aldrich CGD1-1KT) according to the manufacturer’s instructions. Cell death was presented as a percentage of the untreated

(16) Black Walnut (Juglans nigra) Extracts Inhibit Proinflammatory Cytokine Production From Lipopolysaccharide-Stimulated Human Promonocytic Cell Line U-937Frontiers in PharmacologySeptember 19, 2019Khanh-Van Ho, Kathy L. Schreiber, Danh C. Vu, Susan M. Rottinghaus, Daniel E. Jackson, Charles R. Brown, Zhentian Lei, Lloyd W. Sumner, Mark V. Coggeshall, Chung-Ho Lincytotoxicity and/or cell loss at the time of supernatant collection. A colorimetric cell viability assay (CGD1-1KT, Sigma-Aldrich) using MTT as the substrate (Mosmann, 1983) was carried out after collection of the

(17) In vitro comparison of liposomal drug delivery systems targeting the oxytocin receptor: a potential novel treatment for obstetric complicationsInternational Journal of NanomedicineApril 17, 2019Susan Hua, Benjamin Vaughan(F9765), methyl-βcyclodextrin (C4555), nocodazole (M1404), cytochalasin D (C8273), MTT-based assay kit (CGD1), and all buffer reagents were from Sigma-Aldrich Co. l-Glutamine, DMEM, Gibco® Antibiotic-Antimycotic

(18) Salvadora persica Extract-laden Jellyfish Collagen Hybrid Constructs for Periodontal Tissue RegenerationJournal of the Turkish Chemical Society, Section A: ChemistryJanuary 31, 2019Yavuz Emre Arslan, İlkim Kantarcıoğludays. Cell proliferation was quantified using MTT assay kit (Cell Growth Determination Kit, MTT based, CGD1, Merck) by following manufacturer’s instructions at a wavelength of 570 nm on days 3, 7 and 14. RESEARCH

(19) Disulfide bond-disrupting agents activate the tumor necrosis family-related apoptosis-inducing ligand/death receptor 5 pathwayCell death discoveryJanuary 1, 2019Wang M, Law ME, Davis BJ, Yaaghubi E, Ghilardi AF, Ferreira RB, Chiang CW, Guryanova OA, Kopinke D, Heldermon CD, et al.(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. MTT assays were carried out according to the manufacturer’s instructions (kit CGD1, Sigma-Aldrich, St. Louis, MO). In vivo tumor studies, histochemical, and immunohistochemical analysis Mice

(20) Pancreatic stellate cells facilitate pancreatic cancer cell viability and invasionOncology LettersDecember 10, 2018Shang‑Long Liu, Shou‑Gen Cao, Ying Li, Bo Sun, Dong Chen, Dong‑Sheng Wang, Yan‑Bing Zhouevaluation. Cell viability was determined using an MTT assay, according to the manufacturer's protocol (kit CGD1, Sigma-Aldrich,). Briefly, cells were grown in media supplements with 10% FBS, harvested using trypsin

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Cell Growth Determination Kit, MTT based from MilliporeSigma

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Cell Growth Determination Kit, MTT based from MilliporeSigma

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